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KMID : 0880220110490050809
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Journal of Microbiology
2011 Volume.49 No. 5 p.809 ~ p.815
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Biochemical properties of an extracellular trehalase from Malbranchea pulchella var. Sulfurea
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Marita Gimenez Pereira
Joao Atilio Jorge
Hector Francisco Terenzi
Maria de Lourdes Teixeira de Moraes Polizeli
Rosa Prazeres Melo Furriel
Luis Henrique Souza Guimaraes
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Abstract
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The thermophilic fungus Malbranchea pulchella var. sulfurea produced good amounts of extracellular trehalase activity when grown for long periods on starch, maltose or glucose as the main carbon source. Studies with young cultures suggested that the main role of the extracellular acid trehalase is utilizing trehalose as a carbon source. The specific activity of the purified enzyme in the presence of manganese (680 U/mg protein) was comparable to that of other thermophilic fungi enzymes, but many times higher than the values reported for trehalases from other microbial sources. The apparent molecular mass of the native enzyme was estimated to be 104 kDa by gel filtration and 52 kDa by SDS-PAGE, suggesting that the enzyme was composed by two subunits. The carbohydrate content of the purified enzyme was estimated to be 19 % and the pi was 3.5. The optimum pH and temperature were 5.0?5.5 and 55° C, respectively. The purified enzyme was stimulated by manganese and inhibited by calcium ions, and insensitive to ATP and ADP, and 1 mM silver ions. The apparent KM values for trehalose hydrolysis by the purified enzyme in the absence and presence of manganese chloride were 2.70±0.29 and 2.58±0.13 mM, respectively. Manganese ions affected only the apparent Vmax, increasing the catalytic efficiency value by 9.2-fold. The results reported herein indicate that Malbranchea pulchella produces a trehalase with mixed biochemical properties, different from the conventional acid and neutral enzymes and also from trehalases from other thermophilic fungi.
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KEYWORD
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trehalase, extracellular trehalase, acid trehalase, neutral trehalase, Malbranchea pulchella
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